A non-viral CRISPR/Cas9 delivery system for therapeutically targeting HBV DNA and pcsk9 in vivo

Barriers and recent advances in non-viral vectors targeting the lungs for cystic fibrosis gene therapy

Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in CFTR genes that affect chloride ion channel. The CF is a good nominee for gene therapy as the asymptomatic carriers are phenotypically normal, and the desired cells are accessible for vector delivery. Gene therapy shows promising effects involving the correction of gene or replacement of the mutant gene with the func...

passivity in waiting for godot and endgame: a psychoanalytic reading

this study intends to investigate samuel beckett’s waiting for godot and endgame under the lacanian psychoanalysis. it begins by explaining the most important concepts of lacanian psychoanalysis. the beckettian characters are studied regarding their state of unconscious, and not the state of consciousness as is common in most beckett studies. according to lacan, language plays the sole role in ...

in vivo transfection study of chitosan-dna-fap-b nanoparticles as a new non viral vector for gene delivery to the lung

Non-viral siRNA delivery into the mouse retina in vivo

BACKGROUND Gene silencing in the retina using RNA interference could open broad possibilities for functional studies of genes in vivo and for therapeutic interventions in eye disorders. Therefore, there is a considerable demand for protocols to deliver siRNA into the vertebrate retina. In this work we explored a possibility to deliver synthetic 21 bp siRNA into the mouse retina after intravitre...

Efficient Non-viral Gene Delivery in Vivo into Synovium

INTRODUCTION:• Gene delivery into joints is considered to become a useful strategy for the treatment of joint diseases. Viral vectors are commonly used because of their high transduction efficacies. Despite of recent improvement of the vectors, the viral vectors are associated with some unsolved problems such as induction of inflammatory responses and undesired generation of replication-compete...